Figure 1.

6-NBDG two-photon microscopy experimental set-up. a, Detail of the cranial window preparation mounted over the barrel cortex. A tungsten wire (50 μm diameter) was inserted close to the imaging area to record the LFP in layer II–III of the barrel cortex. Agarose filled the chamber to stabilize the brain from cardiorespiratory movements. Astrocytes were labeled using SR-101. b, Characterization of 6-NBDG two-photon excitation in vivo. c, Experimental arrangement for simultaneous LFP recording and two-photon imaging during whisker stimulation. 6-NBDG was infused to the femoral vein. The whiskers of the contralateral side were mechanically stimulated by air puffs generating sustained network activity in the LFP. The time-frequency spectrogram of the LFP showed an increase in the LFP power in the 7–20 Hz range during each period of 30 s of whisker stimulation.