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IKKβ-siRNA inhibits the expression of IKKβ at both the mRNA and protein levels in vitro. A: mRNA transcription of IKKβ in monkey Tenon’s fibroblasts assessed by real-time RT–PCR 24 h after 5–100 nM IKKβ-siRNA were transfected. The normalized IKKβ mRNA level of nontransfected fibroblasts (control) is taken as 1.0 (the asterisk indicates a p<0.05, mean±SD, n=3). B: Protein levels of IKKβ are demonstrated by western blot.
