FIGURE 4.

Ag availability during the CD8⁺ T cell response to infection regulates CD62L expression within the MPEC population. C57BL/6 mice were treated i.p. with either 50 μg or 250 μg of the 25-D1.16 Ab or 250 μg of a control Ab (MOPC-21), after which the mice were infected i.v. with VSV-OVA (A–C) or with LM-OVA (D). At 7 d, the magnitude and phenotype of the OVA/K^b-specific (A, D) or VSV-N/K^b-specific (B) CD8⁺ T cells were monitored in the spleen. Furthermore, 42 d later, mice were sacrificed, and the magnitude and phenotype of the OVA/K^b-specific CD8⁺ T cells were monitored in the spleen (C). Dot plots are gated on CD8⁺ T cells. Values represent the group mean ± one SD. The bar graphs are representations of CD62L expression on the CD127^high KLRG1^low (MPEC) Ag-specific CD8⁺ T cells. Data are representative of five mice per group and two independent experiments. Similar data were also observed in the lymph nodes. Statistical significance was determined by a one-way ANOVA analysis. ***p < 0.001; **p < 0.01; *p < 0.05.