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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Biochem Pharmacol. 2010 Sep 30;81(1):104–110. doi: 10.1016/j.bcp.2010.09.015

Figure 3. Cytotoxicity of an LHRH sequence to PC-3 cells.

Figure 3

(A) PC-3 cells were exposed to a modified LHRH sequence. The data shown represent the average of three individual experiments performed in quadruplicate. Cell viability was determined by MTT. Mean ± s.d. (n=3). (B). PC-3 cells were treated with siRNA-LHRH at 50nM and 100nM. Immunofluorescence was preformed utilizing anti-LHRH antibody with Alexa 488 secondary antibody. (C). PC-3 cells were treated with JC21LHRH or JCHLHRH in the presence or absence of 100nM siRNA-LHRH or siRNA-scramble control. Cell viability was determined by trypan blue exclusion The values are respresentative of three independent experiments (n=3).