Table 1. Summary of proteins with a signal peptide identified by 2D-DIGE and mass spectrometry.

Spots of interest were excised and proteins were identified by mass spectrometry analysis. Proteins were identified by searching the data against the EPICDB database (http://toro.aecom.yu.edu/biodefense/) using MASCOT and are based on two or more peptide sequences with scores above the 95% confidence threshold. U denotes a protein which abundance is higher (up) in the Δsub1 strain; N a protein which abundance is unchanged (neutral) in the Δsub1 strain and D a protein less abundant (down) in the Δsub1 strain. MW: Molecular Weight; pI: isoelectric point. SRS: SAG related sequence. Ca: calcium. Proteins were identified using ToxoDB.org Release 4 gene predictions. Release 5 gene identification numbers for GT-1, a Type I strain like RH are also shown.

Spot	Protein	Gene ID Release 4.3 http://www.toxodb.org	Gene ID Release 5	MW kDa	pI
U1	PLP1	20.m03849	TGGT1_016570	120	4.5
U2	MIC4	25.m00006	TGGT1_021130	63	5.1
U4	SRS	641.m03153	TGGT1_121850	37	4.5
N1	Ca binding	44.m00001	TGGT1_118190	35	4.3
N2	Ca binding	44.m00001	TGGT1_118190	35	4.3
N3	MIC1	80.m00012	TGGT1_031650	60	5.5
N4	AMA1	55.m00005	TGGT1_076680	60	5.9
D1	MIC2	20.m00002	TGGT1_019450	90	4.9
D3	MIC4	25.m00006	TGGT1_021130	63	5.1
D4	MIC4	25.m00006	TGGT1_021130	63	5.1
D6	MIC1	80.m00012	TGGT1_031650	28	4.5
D7	GRA7	20.m00005	TGGT1_017550	22	4.5
D8	SUB1 propeptide	20.m00387	TGGT1_016650	16	4.4
D9	PLP1	20.m03849	TGGT1_016570	115	5.4
D10	SUB1	20.m00387	TGGT1_016650	82	5.1
D16	SRS2	44.m00010	TGGT1_113990	39	7
D18	MIC3	641.m00002	TGGT1_121730	35	5.5
D19	MIC3	641.m00002	TGGT1_121730	30	5.6
D21	MIC4	25.m00006	TGGT1_021130	20	5.6
D22	Hypothetic Protein	31.m00928	TGGT1_039470	17	5.2
D23	SUB1	20.m00387	TGGT1_016650	82	5.1
D26	MIC3	641.m00002	TGGT1_121730	38	6.1