Fig. 4.

The effect of visnagin on the GFAP, OX-42 expression induced by KA in hippocampal CA3 region. OX-42 and GFAP immunoreactivities (A) in the hippocampus were examined at 24 hr after i.c.v. injection of KA (0.1 µg/5 µl). Animals (N=5 per each group) were pretreated orally with either PBS or visnagin (100 mg/kg) 20 min prior to KA (0.1 µg/5 µl). To quantify, we counted GFAP or OX-42 IR in each section (B). CON (a): vehicle (i.p.)+PBS (i.c.v.), KA (b): vehicle (i.p.)+KA (i.c.v.), Vis (c): Visnagin (i.p.)+PBS (i.c.v.), Vis+KA (d): Visnagin (i.p.)+KA (i.c.v.). Antibody against OX-42 and GFAP was used at 1:10,000 dilution for immunostaining. ^*p<0.05 (KA vs Vis+KA), ^**p<0.01 (KA vs Vis+KA).