Fig. 7.

Effects of PcrA and Rep on post-incision NER complexes. (A) UvrC-turnover experiments were performed using 1.2 nM UV-irradiated plasmid DNA, 6 nM UvrA, 30 nM UvrB, 3 nM UvrC and indicated concentrations of helicases in repair buffer. Values shown are the average of 3 independent experiments, with standard deviation. (B) Analysis of DNA cleavage in post-incision complexes incubated with or without helicases. Samples were analysed as in Fig. 3C. (C) Displacement of the excised oligonucleotide from a post-incision complex in the presence or absence of helicases. Samples were analysed as in Fig. 3D. The gels shown are representative of at least 3 independent experiments.