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. Author manuscript; available in PMC: 2010 Dec 6.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jul 17;374(3):479–484. doi: 10.1016/j.bbrc.2008.07.042

Fig. 3.

Fig. 3

Effect of TGF-β1 on (A) VEGF-A and (B) sVEGF-R1 secretion by HCRF cells and its inhibition by TGF-β receptor 1 kinase inhibitor. HCRF cells were incubated with TGF-β1(10 ng/ml) for 24 h in serum free medium. For inhibitor studies, cultures were pretreated with the inhibitor for 30 min. Culture supernatants were used for analysis of VEGF-A and sVEGF-R1 by ELISA. Results are means ± SD from one representative experiment with triplicate samples. Effect of IFN-γ on TGF-β1 and TGF-β2 induced VEGF-A (C) and sVEGF-R1 (D) secretion by HCRF cells. Cultures were incubated with IFN-γ (100 U/ml) and TGF-β (10 ng/ml) in serum free medium for 24 h. Levels of VEGF-A and sVEGF-R1 in culture supernatants were determined by ELISA. Results are means ± SE for five experiments each with duplicate samples. *p < 0.01.