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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Nov 12;391(1):287–292. doi: 10.1016/j.bbrc.2009.11.051

Fig. 3.

Fig. 3

JAK-STAT inhibitor reverses IFN-γ inhibition of TNF-α + IL-1β induced IL-11 secretion by HRPE (A) and HCRF (B) cells. Cultures were pre-incubated with JAK inhibitor 1 for 30 min before treating with TNF-α + IL-1β for 24 h in SFM. The levels of IL-11 in culture supernatants were determined by ELISA. *p < 0.001 for inhibition by IFN-γ. @p < 0.01 for reversal of IFN-γ inhibition by JAK-1 inhibitor. TGF-β1 receptor inhibitor abolishes TGF-β1 induced IL-11 secretion by HRPE (C) and HCRF (D). The cultures were pre-incubated with the indicated inhibitors for 30 min before treating with TGF-β1 for 24 h in SFM. Levels of IL-11 in the culture supernatants were determined by ELISA. *p < 0.001 for inhibition of TGF-β1 effects by TGF-β receptor inhibitors. The results are means ± SEM of 4 experiments each performed with duplicate samples.