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(A) Changes in single-molecule FRET histograms during the opening of the DNA quadruplex by hybridization to 7 nM III. The “zero” peak at t = 0 has been subtracted from all histograms for clarity. The experiment was carried out at 20°C in 100 mM NaCl and 10 mM Tris·HCl (pH 7.4). (B) Kinetic traces for the low- and high-FRET subpopulations. Data were fit to a single exponential, y = A e–t/τ + C.
