Table 2.

Utility of the two DBS real-time PCR assays to identify infants with confirmed congenital CMV infection.

	Single-primer DBS PCR			Two-primer DBS PCR
Congenital CMV Infection	+	−	Total	+	−	Total
+	17	43	60	11	21	32
−	4	11,343	11,347	1	8,985	8,986
Total	21	11,386	11,407	12	9,006	9,018
Sensitivity	28.3% (17.4 – 41.4%)			34.4% (18.6 – 53.2%)
Specificity	99.9% (99.9 – 100%)			99.9% (99.9 – 100%)
Positive Likelihood Ratio	803.7 (278.7 – 2,317.9)			3088.9 (410.8 – 23,226.7)
Negative Likelihood Ratio	0.7 (0.6 – 0.8)			0.7 (0.5 – 0.8)
Positive Predictive Value	80.9% (58.1 – 94.5%)			91.7% (61.5 – 99.8%)
Negative Predictive Value	99.6% (99.5 – 99.7%)			99.8% (99.6 – 99.9%)

DBS indicates dried blood spots; PCR, polymerase chain reaction; CMV, cytomegalovirus