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. Author manuscript; available in PMC: 2010 Dec 6.
Published in final edited form as: Brain Res. 2009 Aug 11;1296:35–45. doi: 10.1016/j.brainres.2009.08.005

Figure 2.

Figure 2

Intracerebroventricular insulin administration increases plasma membrane association of GLUT4 in the rat hippocampus. Panel A. Representative immunoblots of GLUT4 immunoreactive bands in hippocampal plasma membrane fractions (PM; top panel) and total membrane fractions (T; bottom panel) 15, 30, 45 and 60 minutes following icv insulin treatment; zero time point rats were injected with saline. Panel B. Autoradiographic image analysis revealed that insulin increased plasma membrane association of GLUT4 in the rat hippocampus at the 15 and 30 minute post-insulin time points, increases that returned to baseline at the 45 minute time point. Molecular weight standards are shown on the left. Data in Panel B are expressed as the plasma membrane GLUT4/total membrane GLUT4 ratio and are based upon at least 6 rats/group. * = p ≤ 0.001 compared to saline control rats; $ = p ≤ 0.001 compared to 45 minute insulin group; # = p ≤ 0.001 compared to 60 minute insulin group.