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Transformation of tobacco by using glyoxalase pathway genes. (A) Schematic representation of various glyoxalase constructs used to overexpress gly I enzyme (pBI-SI) and gly II enzyme (pCAM-glyII) in tobacco plants. (B) Testing of various gly II (GII) and double (GI+II) transgenic lines for the presence of gly II transgene by Southern hybridization. The line number of each type of transgenic is given at the top. The presence of a 1.0-kb band on the Southern blot is shown by an arrowhead.
