Relative salt tolerance of WT and glyoxalase-overexpressing transgenic T1 generation tobacco plants (GI, gly I; GII, gly II; and GI+II, double transgenics) at seedling and whole mature plant level. (A) Seedlings were grown on medium supplemented with 0, 100, 200, and 400 mM NaCl for 25 days. (B) WT and transgenic plants were grown in the continued presence of 200 mM NaCl for 98 days. Note that WT plants could not sustain growth under this condition.