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(A) RT-PCR analysis of MUC17 expression in various colon cancer cell lines. Actin was used as an internal control. Except for LS174T and LS-180 cells, none of the colon cancer cells have a detectable expression of MUC17. (B) Protein lysates from all the colon cancer cell lines and the AsPC-1 pancreatic cancer cell line (as a positive control), were resolved on 2% agarose gel. The MUC17 protein was only detected in LS174T and LS-180 cells. β-actin was used as a loading control.
