Figure 2.

Bioluminescence imaging (BLI) of transgene expression after rAAV2/6.2-mediated gene delivery in fetal lung. (a,b) Low-dose rAAV2/6.2 vectors encoding β-gal [2 × 10⁹ genome copies (GC)/fetus] or fLuc (2 × 10⁸ GC/fetus) were co-injected i.t. or i.a. in fetal Naval Medical Research Institute mice at E18 and followed up by noninvasive BLI at 1 and 4 weeks after injection. (c) Quantification of total photon flux after low-dose rAAV2/6.2. (d,e) BLI signal at 1 and 4 weeks after injection after high-titer rAAV2/6.2 (3 × 10¹⁰ GC/fetus for β-gal and fLuc) administration with corresponding quantification of (f) total photon flux. All animals were scanned, separated by black partitions, to avoid scattering of photons to neighboring animals. The pseudocolor scale depicts the photon flux per second, per square centimeter per steradian (p/s/cm²/sr). Measurements were obtained in a 4.3 cm² rectangular region of interest. Please note that the scales of the BL images are different between the time points. Mean ± SD, analysis of variance, Student's t-test, *P < 0.05, **P < 0.01, ***P < 0.001. neg., negative.