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. 2010 Jul 27;18(12):2130–2138. doi: 10.1038/mt.2010.153

Figure 5.

Figure 5

rAAV2/6.2-mediated transgene expression in murine airway epithelium after fetal intratracheal injection. β-gal gene transfer to the fetal mouse lung was assessed by X-gal staining. Sections were counterstained with paracarmine. Transduction efficiency is illustrated after high-dose rAAV2/6.2 vector administration (3 × 1010 GC/fetus) at weeks 1 and 4. Representative images of different lung regions are given at 1 and 4 weeks after fetal i.t. injection, respectively, showing the trachea, the bronchi, the bronchioles, and the alveoli. A high magnification image depicts β-gal positive ciliated (bottom left image) and alveolar cells (bottom middle image) at week 1. Absence of reporter gene expression in a control lung (bottom right image). Bar = 50 µm.