Figure 2.

Expression of mutTGFβ3 retards wound closure of in vitro scrape assays in murine dermal fibroblasts (MDFs). (a) MDF cells were transduced with lentivectors expressing either wild-type or mutant (C25G LAP) TGFβ3 or GFP control (10 multiplicity of infection) then seeded on plastic and grown to 90% confluence. A single midline scrape was made in each well and real-time imaging carried out every 10 minutes over 48 hours full data stream is shown in Supplementary Movie S1A. (b) Migration rates for each group were derived by performing linear regression on the data set from each well (n = 3 ± SEM). mutTGFβ3, mutant transforming growth factor β3.