Figure 3.

mutTGFβ3 retardation of wound closure is mediated by integrin. (a) Scrape assays were set up with the VB6 cell line that overexpresses β6 VB6 integrin compared to their parental line C1. Cells were transduced with lentivectors expressing wild-type or mutant TGFβ3 or GFP control. Imaging was carried out as described over 48 hours (Supplementary Movie S1B). (b) Migration rates for each group were derived by performing linear regression on the data set from each well (n = 2–3 ± SEM). GFP, green fluorescent protein; mutTGFβ3, mutant transforming growth factor β3.