Figure 2.

Screenshot of GATMD ‘Search’ page. The topology diagram of GAT1 shown corresponds to the sequence alignment of Yamashita et al. (41). Out and In refer to the extracellular and intracellular space, respectively. Cylinders (numbered 1–12) correspond to alpha helical, membrane-spanning regions. Transmembrane domains 1 and 6 contain unwound regions as suggested by the crystal structure of the related bacterial leucine transporter, LeuT_Aa (41). Each residue is depicted by its one-letter amino acid symbol. For each of the 599 residues, a hyperlink is provided, which will retrieve all database experimental records associated with that residue. Additional search features of the database may be accessed under the ‘Basic Search Functions’ and ‘Advanced Search Functions’ menus. A tutorial is also available.