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. Author manuscript; available in PMC: 2011 Nov 26.
Published in final edited form as: Circ Res. 2010 Oct 14;107(11):1336–1344. doi: 10.1161/CIRCRESAHA.110.227926

Figure 4. miR-218 contributes to EC migration.

Figure 4

A. Real Time RT-PCR reveals knockdown or overexpression of miR-218 in HUVECs transfected with 2-O’Me modified antisense or mimic oligonucleotides. B. Images of scratch wound following ~12 hours of incubation. MS1 cells (top row) or HUVECs (bottom row) were transfected with control, anti miR-218 or miR-218 mimic for 48 hours prior to scratch assay. Dotted lines denote front of EC migration. C, E. Quantification of migration in MS1 ECs (D) or HUVECs (E). Y-axis is the width of the “wound” 12 hours after scratching. Measurements are taken at 4 locations per well in duplicate wells in 3 independent experiments. Error bars represent SEM.