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. Author manuscript; available in PMC: 2011 Nov 26.
Published in final edited form as: Circ Res. 2010 Oct 14;107(11):1336–1344. doi: 10.1161/CIRCRESAHA.110.227926

Figure 5. miR-218 modulates expression of Slit-Robo axis genes in the retina.

Figure 5

A. Experimental setup of retinal injections. Postnatal (P) age is denoted above line. Timepoints of injection and sample isolation are denoted below line. B. Real Time RT-PCR demonstrates knockdown of miR-218 or miR-126 in retinal samples 3 days after injection with LNA modified antisense miR-218 or miR-126 oligonucleotides. Expression is relative to control oligonucleotide and normalized to U6. C. Real time RT-PCR reveals expression levels of GLCE and Robo1 from retinal samples 3-days after injection. Error bars are SEM (n = 3, * denotes p < 0.05). D. Western for Robo1, Robo2, and GLCE from a pool of 5 retinas, 3 days after injection with control or anti miR-218 oligonucleotides. GAPDH is detected as loading control. C = control, 218 = anti miR-218. E. Quantification of protein levels from retinal explant pools. F. Western blot detecting Robo1 and GLCE from retinal ECs 48 hrs after transfection with anti miR-218 oligonucleotides. GAPDH is detected as loading control. Numbers refer to Western band intensity, normalized to GAPDH and relative to control.