Figure 3.

Late-phase lipopolysaccharide (LPS) preconditioning made no difference in the phosphorylation of Akt, but inhibited the activation of transcription factor nuclear factor (NF)-κB. Mice underwent 100 µg/kg LPS pretreatment 20 h before 90 min ischaemia and reperfusion. Samples were obtained 1 h after reperfusion (n = 6). (A) Effect of LPS preconditioning on the phosphorylation of Akt was examined by Western blotting. (B) NF-κB binding activity was investigated by electrophoretic mobility shift assay using nuclear extracts prepared from liver samples. LPC(−), LPS-non-treated group; Late-LPC, late-phase LPS preconditioning group; Phospho-, phosphorylated