Figure 6.

Lipopolysaccharide (LPS) preconditioning induced suppressor of cytokine signalling-1 (SOCS-1) in the earlier post-ischaemic period. Mice were subjected to pretreatment, ischaemia and reperfusion as described above. Protein extracts were prepared from liver parenchyma sampled after reperfusion (n = 5). Expression of SOCS-1 was investigated using (A) reverse transcription polymerase chain reaction and (B) Western blotting. As an endogenous control, the amplification of 18S rRNA was used. Results are expressed as mean ± standard error of the mean. *P < 0.05, compared with the LPC(−) group. LPC(−), LPS-non-treated group; Late-LPC, late-phase LPS preconditioning group