Table 1.
Activities of recombinant GSTs from various species
| Enzyme substrate | PcpF | PcpF C53S | Re-YqjG | Ec-YqjG | ECM4 | hGSTO1-1 | hGSTO2-2 |
|---|---|---|---|---|---|---|---|
| CDNB | ND | ND | ND | ND | ND | 0.02 ± 0.01 | 0.01 ± 0.01 |
| Ethacrynic acid | 0.09 ± 0.01 | 0.11 ± 0.01 | 0.26 ± 0.05 | 0.07 ± 0.01 | 0.13 ± 0.01 | 0.02 ± 0.01 | 0.37 ± 0.06 |
| GS-TriCH | 2.66 ± 0.12 | ND | 1.67 ± 0.13 | 1.76 ± 0.07 | 3.18 ± 0.06 | ND | ND |
| HED | 23.1 ± 0.8 | ND | 37.9 ± 0.8 | 9.54 ± 0.23 | 22.9 ± 0.3 | 3.91 ± 0.09 | 9.73 ± 0.08 |
| DMAV | 2.49 ± 0.31 | ND | 2.28 ± 0.13 | 1.30 ± 0.24 | 1.42 ± 0.17 | 0.074 ± 0.008 | 0.46 ± 0.02 |
| DHA | 1.91 ± 0.14 | ND | 1.71 ± 0.16 | 0.27 ± 0.03 | 0.88 ± 0.02 | 0.57 ± 0.01 | 26.5 ± 0.53 |
| DiCPGS | 0.22 ± 0.01 | ND | ND | ND | ND | 1.56 ± 0.01 | ND |
Specific activities are reported as substrate consumed (μmol · min−1 · mg−1) of protein at 23 ± 1 °C and are means ± S.D. Assays were performed with 5 mM GSH except for the ethacrynic acid assay which used 1 mM GSH. ND, no apparent activity was detected; DiCPGS, S-(2′4′-dichlorophenacyl)glutathione.