Figure 7.

Downregulation of caspase-8 prevents TRAIL-induced DNA damage. MEF cells were transfected with the indicated concentrations of siRNAs targeting caspase-8 or glyceraldehyde 3-phosphate dehydrogenase then subjected to immunoblotting (a) or treated with 300 ng/ml cross-linked TRAIL and assayed for H2AX phosphorylation (b). The left Y-axis and black lines represent the percentage of viable cells. The right Y-axis and gray lines show the proportion of cells bearing phosphorylated H2AX. Error bars indicate s.e.m. from three independent experiments.