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. 2010 Jul 5;29(36):5032–5047. doi: 10.1038/onc.2010.250

Figure 5.

Figure 5

ARRDC3 negatively regulates ITGβ4. (a) Western blot analysis shows that ITGβ4 protein level is affected by ARRDC3 expression. Stable alterations of ARRDC3 expression in MDA-MB-231, MDA-MB-435 and MDA-MB-435+β4 cell lines. When ARRDC3 expression was repressed using two separate short hairpin RNA constructs (shA or shC), cells showed an increase in ITGβ4 protein when compared with control cells (Un or shCtl). Conversely, when cells were made to overexpress ARRDC3 (ARRDC3), ITGβ4 levels decreased when compared with vector control cells (Flag) or untransfected parental cells (Un). (b) Immunofluorescence shows that when MDA-MB-231 cells overexpress ARRDC3 using a green fluorescent protein (GFP)-expressing adenovirus (adARRDC3), ITGβ4 levels decrease when compared with uninfected cells (no GFP expression) and cells infected with a control GFP-expressing adenovirus (adGFP).