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The regulation of ITGβ4 by ARRDC3 is specific and not a product of generalized increased endocytosis. MDA-MB-231 cells were infected with either a green fluorescent protein (GFP)-expressing adenovirus (+GFP) or an ARRDC3-expressing adenovirus (+ARRDC3). Nonpermeabilized cells were then analyzed by flow cytometry for various cell surface proteins. ARRDC3 overexpression did not affect surface levels of ITGβ1, CD44 or EpCam.
