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. Author manuscript; available in PMC: 2011 Mar 30.
Published in final edited form as: Oncogene. 2010 Jul 19;29(39):5359–5369. doi: 10.1038/onc.2010.274

Fig. 3. PRL-induced mammary cancer cells that are deficient in Jak2 lack activation of Stat5.

Fig. 3

(a) Experimental design for the conditional deletion of Jak2 in PRL-induced mammary cancer cells. (b) PCR assay to verify the loss of both Jak2 floxed alleles (Jak2fl/fl) and the presence of Jak2 knockout alleles (Jak2-/-) following Cre-mediated recombination. (c) Expression analysis of Jak2, Estrogen Receptor α (ERα), and Cyclin D1 (Ccnd1) in Jak2-deficient cancer cells and their isogenic control cells. Beta-Actin (ActB) served as a loading control. (d) Immunoprecipitation (IP) and western blot (WB) analysis to assess the activation of Stat5 and Stat3 following PRL stimulation.