Table 2. Proteins identified only in the beta cells.
Ontology | Symbol | Gene name | percentile | molar amount |
(%) | GEOnorm | |||
Endoplasmic reticulum, Golgi apparatus and secretory vesicles | ||||
Gosr2 | golgi SNAP receptor complex member 2 | 90 | 0.8865 | |
Copb2 | coatomer protein complex, subunit beta 2 (beta prime) | 70 | 0.3747 | |
Ufm1 | ubiquitin-fold modifier 1 | 65 | 0.3604 | |
Pcsk1 | proprotein convertase subtilisin/kexin type 1 | 65 | 0.3285 | |
Sytl4 | synaptotagmin-like 4 | 60 | 0.3080 | |
Sec31a | SEC31 homolog A (S. cerevisiae) | 55 | 0.2466 | |
Sec13 | SEC13 homolog (S. cerevisiae) | 55 | 0.2414 | |
Uso1 | USO1 homolog, vesicle docking protein (yeast) | 50 | 0.2209 | |
Prrc1 | proline-rich coiled-coil 1 | 40 | 0.1830 | |
Bnip1 | BCL2/adenovirus E1B 19 kDa-interacting protein 1 | 30 | 0.1268 | |
Gorasp2 | golgi reassembly stacking protein 2 | 30 | 0.1327 | |
Nsfl1c | NSFL1 (p97) cofactor (p47) | 20 | 0.0960 | |
Nsfl1c | NSFL1 (p97) cofactor (p47) | 20 | 0.0960 | |
Rnpep | arginyl aminopeptidase (aminopeptidase B) | 15 | 0.0768 | |
Intracellular signaling | ||||
Adrbk1 | Adrenergic, beta, receptor kinase 1 | 90 | 1.1449 | |
Drd3 | dopamine receptor D3 | 90 | 1.2612 | |
Tpt1 | tumor protein, translationally-controlled 1 | 80 | 0.5835 | |
Ppp1r1a | protein phosphatase 1, regulatory (inhibitor) subunit 1A | 65 | 0.3683 | |
Gpsm1 | G-protein signaling modulator 1 (AGS3-like, C. elegans) | 55 | 0.2456 | |
Map2k1 | mitogen activated protein kinase kinase 1 | 30 | 0.1378 | |
Camk2d | calcium/calmodulin-dependent protein kinase II delta | - | nq | |
Camk2a | calcium/calmodulin-dependent protein kinase II alpha | - | nq | |
Camk2g | calcium/calmodulin-dependent protein kinase II gamma | - | nq | |
Metabolism | ||||
Echs1 | enoyl Coenzyme A hydratase, short chain, 1, mitochondrial | 65 | 0.3315 | |
Echdc1 | enoyl Coenzyme A hydratase domain containing 1 | 60 | 0.2786 | |
Ddc | dopa decarboxylase (aromatic L-amino acid decarboxylase) | 60 | 0.2903 | |
Gpd2 | glycerol-3-phosphate dehydrogenase 2, mitochondrial | 55 | 0.2506 | |
Aco1 | aconitase 1, soluble | 50 | 0.2151 | |
Cox5b | cytochrome c oxidase subunit Vb | 50 | 0.2205 | |
Aldh7a1 | alpha-amino adipic semialdehyde dehydrogenase (antiquitin) | 45 | 0.2076 | |
Atp6v1e1 | ATPase, H+ transporting, lysosomal V1 subunit E1 | 45 | 0.2012 | |
Slc2a2 | facilitated glucose transporter 2 | 35 | 0.1532 | |
Aldh6a1 | methylmalonate semialdehyde dehydrogenase | 30 | 0.1386 | |
Ddt | D-dopachrome tautomerase | 30 | 0.1347 | |
Acaa1a | acetyl-Coenzyme A acyltransferase 1 (peroxisomal) | 25 | 0.1023 | |
Sardh | sarcosine dehydrogenase | 25 | 0.1074 | |
Acads | acyl-Coenzyme A dehydrogenase, short chain | 15 | 0.0716 | |
Cell motility and cytoskeleton | ||||
Vcl | vinculin | 50 | 0.2149 | |
Wdr1 | WD repeat domain 1 | 50 | 0.2195 | |
Dctn2 | dynactin 2 | 45 | 0.2066 | |
Dcx | doublecortin | 25 | 0.1123 | |
Tpm4 | tropomyosin 4 | 20 | 0.0940 | |
Fis1 | fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) | 20 | 0.0954 | |
Capzb | capping protein (actin filament) muscle Z-line, beta | 10 | 0.0644 | |
Tpm1 | tropomyosin 1, alpha | 5 | 0.1831 | |
Proteasome | ||||
Psmc3 | proteasome (prosome, macropain) 26S subunit, ATPase 3 | 45 | 0.1946 | |
Psmc2 | proteasome (prosome, macropain) 26S subunit, ATPase 2 | 20 | 0.0858 | |
Psmc5 | proteasome (prosome, macropain) 26S subunit, ATPase, 5 | 15 | 0.0777 | |
Protein synthesis - translation | ||||
Wars | tryptophanyl-tRNA synthetase | 40 | 0.1799 | |
Gars | glycyl-tRNA synthetase | 40 | 0.1657 | |
Tars | threonyl-tRNA synthetase | 30 | 0.1275 | |
Asns | asparagine synthetase | 20 | 0.0988 | |
Sars | seryl-tRNA synthetase | 15 | 0.0673 | |
6 reference proteins present in all tissues and used for GEOnormalization | ||||
Tubb5 | tubulin, b5 | - | 2.0417 | |
Ppia | peptidylprolyl isomerase A (cyclophilin A) | - | 2.0273 | |
Hspa8 | heat shock protein 8 | - | 1.2830 | |
Ywhae | 14-3-3 protein epsilon | - | 1.0002 | |
Pfn1 | profilin 1 | - | 0.7672 | |
Rab1b | RAB1B, member RAS oncogene family | - | 0.2454 |
93 proteins were only identified in the beta cells. Table 2 shows a selection of proteins, from statistically overrepresented (p<0.005) functional ontologies. Percentile (%) score ranks the proteins molar abundance (not normalized) to those of all other 943 identified proteins in tissue-comparative analysis with 100% = highest abundance. GEOnorm molar amount represents measured molar units, normalized to the 6 stably expressed proteins that are shown as reference at bottom of the table.