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. 2010 Dec 6;5(12):e14214. doi: 10.1371/journal.pone.0014214

Table 2. Proteins identified only in the beta cells.

Ontology Symbol Gene name percentile molar amount
(%) GEOnorm
Endoplasmic reticulum, Golgi apparatus and secretory vesicles
Gosr2 golgi SNAP receptor complex member 2 90 0.8865
Copb2 coatomer protein complex, subunit beta 2 (beta prime) 70 0.3747
Ufm1 ubiquitin-fold modifier 1 65 0.3604
Pcsk1 proprotein convertase subtilisin/kexin type 1 65 0.3285
Sytl4 synaptotagmin-like 4 60 0.3080
Sec31a SEC31 homolog A (S. cerevisiae) 55 0.2466
Sec13 SEC13 homolog (S. cerevisiae) 55 0.2414
Uso1 USO1 homolog, vesicle docking protein (yeast) 50 0.2209
Prrc1 proline-rich coiled-coil 1 40 0.1830
Bnip1 BCL2/adenovirus E1B 19 kDa-interacting protein 1 30 0.1268
Gorasp2 golgi reassembly stacking protein 2 30 0.1327
Nsfl1c NSFL1 (p97) cofactor (p47) 20 0.0960
Nsfl1c NSFL1 (p97) cofactor (p47) 20 0.0960
Rnpep arginyl aminopeptidase (aminopeptidase B) 15 0.0768
Intracellular signaling
Adrbk1 Adrenergic, beta, receptor kinase 1 90 1.1449
Drd3 dopamine receptor D3 90 1.2612
Tpt1 tumor protein, translationally-controlled 1 80 0.5835
Ppp1r1a protein phosphatase 1, regulatory (inhibitor) subunit 1A 65 0.3683
Gpsm1 G-protein signaling modulator 1 (AGS3-like, C. elegans) 55 0.2456
Map2k1 mitogen activated protein kinase kinase 1 30 0.1378
Camk2d calcium/calmodulin-dependent protein kinase II delta - nq
Camk2a calcium/calmodulin-dependent protein kinase II alpha - nq
Camk2g calcium/calmodulin-dependent protein kinase II gamma - nq
Metabolism
Echs1 enoyl Coenzyme A hydratase, short chain, 1, mitochondrial 65 0.3315
Echdc1 enoyl Coenzyme A hydratase domain containing 1 60 0.2786
Ddc dopa decarboxylase (aromatic L-amino acid decarboxylase) 60 0.2903
Gpd2 glycerol-3-phosphate dehydrogenase 2, mitochondrial 55 0.2506
Aco1 aconitase 1, soluble 50 0.2151
Cox5b cytochrome c oxidase subunit Vb 50 0.2205
Aldh7a1 alpha-amino adipic semialdehyde dehydrogenase (antiquitin) 45 0.2076
Atp6v1e1 ATPase, H+ transporting, lysosomal V1 subunit E1 45 0.2012
Slc2a2 facilitated glucose transporter 2 35 0.1532
Aldh6a1 methylmalonate semialdehyde dehydrogenase 30 0.1386
Ddt D-dopachrome tautomerase 30 0.1347
Acaa1a acetyl-Coenzyme A acyltransferase 1 (peroxisomal) 25 0.1023
Sardh sarcosine dehydrogenase 25 0.1074
Acads acyl-Coenzyme A dehydrogenase, short chain 15 0.0716
Cell motility and cytoskeleton
Vcl vinculin 50 0.2149
Wdr1 WD repeat domain 1 50 0.2195
Dctn2 dynactin 2 45 0.2066
Dcx doublecortin 25 0.1123
Tpm4 tropomyosin 4 20 0.0940
Fis1 fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) 20 0.0954
Capzb capping protein (actin filament) muscle Z-line, beta 10 0.0644
Tpm1 tropomyosin 1, alpha 5 0.1831
Proteasome
Psmc3 proteasome (prosome, macropain) 26S subunit, ATPase 3 45 0.1946
Psmc2 proteasome (prosome, macropain) 26S subunit, ATPase 2 20 0.0858
Psmc5 proteasome (prosome, macropain) 26S subunit, ATPase, 5 15 0.0777
Protein synthesis - translation
Wars tryptophanyl-tRNA synthetase 40 0.1799
Gars glycyl-tRNA synthetase 40 0.1657
Tars threonyl-tRNA synthetase 30 0.1275
Asns asparagine synthetase 20 0.0988
Sars seryl-tRNA synthetase 15 0.0673
6 reference proteins present in all tissues and used for GEOnormalization
Tubb5 tubulin, b5 - 2.0417
Ppia peptidylprolyl isomerase A (cyclophilin A) - 2.0273
Hspa8 heat shock protein 8 - 1.2830
Ywhae 14-3-3 protein epsilon - 1.0002
Pfn1 profilin 1 - 0.7672
Rab1b RAB1B, member RAS oncogene family - 0.2454

93 proteins were only identified in the beta cells. Table 2 shows a selection of proteins, from statistically overrepresented (p<0.005) functional ontologies. Percentile (%) score ranks the proteins molar abundance (not normalized) to those of all other 943 identified proteins in tissue-comparative analysis with 100%  =  highest abundance. GEOnorm molar amount represents measured molar units, normalized to the 6 stably expressed proteins that are shown as reference at bottom of the table.