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. 2010 Dec 6;5(12):e14239. doi: 10.1371/journal.pone.0014239

Figure 3. RNA interference of oleate desaturase expression in procyclic-form trypanosomes.

Figure 3

Growth curves (logarithmic plot) of procyclic-form cell line 29-13 wild-type cells (wt PCF) and cells transfected with a construct for RNAi of OD. Cells of the transfected clone were cultured in the absence (-tet) and presence of tetracycline (+tet) to induce RNA interference (A). Relative levels of OD mRNA, normalized to tubulin mRNA levels. The results are expressed as percentage of the normalized OD transcripts present in the untransfected control (wt) cells and are the means ± SD of three independent experiments (B).