Figure 6. Effect of 13-thiastearate on the incorporation of radioactivity in membrane lipids of bloodstream-form Trypanosoma brucei from radiolabeled fatty acids.

BSF cells were preincubated for 30 min with 50 µM 13-TS, then incubated with [¹⁴C]-oleate (A) or [¹⁴C]-stearate (B) for 1 h. The lipids were extracted by phase partitioning and fatty acids were analyzed by argentation-TLC after transmethylation, as described in Materials and Methods. The radioactive spots were scraped from the plate and quantified by liquid scintillation radioassay. The abundance of each fatty acid is presented as the percentage of sum of oleate (C18:1) and linoleate (C18:2) (A) or the sum of stearate (18:0), C18:1 and C18:2 (B). The results are means ± SD of three independent experiments.