Figure 2. Swe1^K594R is more stable and expressed in higher levels then wild type Swe1.

A. Swe1 activity is more persistent with a delay in Cdc5 accumulation and destruction of Swe1 in swe1^K594R cells. W303a cells were arrested for 2 hours with 0.2 M hydroxyurea and released into fresh media containing 5 µg/ml nocodazole. B. Swe1 activity is more persistent with a delay in Cdc5 accumulation and destruction of Swe1 when swe1^K594R -3myc is expressed from a GAL promoter for 1 hour in cdk1^as1 swe1Δ cells. Cells were released into media containing 5 µg/ml nocodazole and 4% glucose to shut-off Swe1-3myc expression. C. Localisation of Swe1-GFP and swe1^K594R-GFP expressed from a GAL promoter for 90 minutes.