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. 2010 Dec 6;5(12):e15089. doi: 10.1371/journal.pone.0015089

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Simpson-Lavy, Brandeis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Swe1^K594R is intrinsically more abundant, even in a cdk1^Y19F background. However, the delay in Swe1 destruction is abolished. Cells were arrested for 2 hours with 0.2 M hydroxyurea and released into fresh media containing 5 µg/ml nocodazole. B. Swe1^K594R is intrinsically more abundant, even when all 18 Cdk1 phosphorylation sites present in Swe1 are mutated. Cells were arrested for 2 hours with 0.2 M hydroxyurea and released into fresh media containing 5 µg/ml nocodazole.