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. 2010 Dec 6;5(12):e15089. doi: 10.1371/journal.pone.0015089

Figure 4. Siz1 is the SUMO ligase of Swe1.

Figure 4

A. Siz1Δ cells exhibit elevated Swe1 levels. JD52° cells were left unsynchronized in mid-log phase (un), or arrested in S-phase with 0.2 M hydroxyurea (HU). B. The 110 kDa SUMO band (Figure 1B) is also absent in siz1Δ cells. C. Accumulation of Cdc5 and destruction of Swe1 is delayed in siz1Δ cells. Swe1-3myc was expressed from a GAL promoter in JD52° cells for 1 hour before being shut-off by addition of glucose. D. Swe1 activity is more persistent with a delay in Cdc5 accumulation and destruction of Swe1 in siz1Δ cells. W303a cells were arrested for 2 hours with 0.2 M hydroxyurea and released into fresh media containing 5 µg/ml nocodazole.