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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: J Am Chem Soc. 2010 Nov 10;132(47):16812–16817. doi: 10.1021/ja105429n

Figure 3.

Figure 3

Visual evaluation of the library at different screening conditions, including sequences identified from a 100 μM PLP reaction. (a) When incubated with 10 mM PLP, the majority of the library members turned red, confirming that at standard reaction conditions many sequences exhibit at least some degree of conversion. (b) Using 1 mM PLP, fewer library members turned red, and most showed a lighter color. (c) Using only 100 μM PLP, most of the sequences showed no conversion (as evidenced by the colorless beads) but a small number of red beads were still identified. (d) The sequences corresponding to the red beads at 100 μM PLP revealed AXK and AKX as the predominant N-terminal motifs.