. Author manuscript; available in PMC: 2011 Dec 6.

Published in final edited form as: Mol Pharm. 2010 Nov 11;7(6):2362–2368. doi: 10.1021/mp100300k

Table 2.

Michaelis-Menten kinetic parameters of valine and phenylalanine derivatives for hVACVase-mediated hydrolysis (mean ± SEM; Phe-OMe, n=2; others, n=3).

valine analogues	K_m (μM)^b	V_max (nmol/min/μg)	k_cat (s^-1)^c	k_cat/K_m (mM^-1·s^-1)
valine p-nitrobenzyl ester	14 ± 1	272 ± 3	130 ± 2	9490 ± 580
Val-3-HPG^a	46 ± 5	321 ± 9	154 ± 4	3370 ± 460
valacyclovir^a	68 ± 4	120 ± 2	58 ± 1	850 ± 66
Val-OBn	60 ± 15	125 ± 7	60 ± 3	992 ± 300
Val-3-APG	1810 ± 80	(4.12 ± 0.08) × 10^-3	(2.29 ± 0.04) × 10^-3	(1.26 ± 0.08) × 10^-3

phenylalanine analogues	K_m (μM)^d	V_max (nmol/min/μg)	k_cat (s^-1)^e	k_cat/K_m (mM^-1·s^-1)
Phe-3-HPG^a	207 ± 16	718 ± 16	345 ± 8	1660 ± 160
Phe-OBn	99 ± 26	822 ± 54	395 ± 26	3980 ± 1310
Phe-OMe	5140 ± 1120	474 ± 49	228 ± 24	44 ± 14
Phe-OEt	6700 ± 560	193 ± 8	93 ± 4	14 ± 2

Previously published by Sun et al.¹²

Statistically different groups: A. valine p-nitrobenzyl ester, Val-3-HPG, valacyclovir, Val-OBn; B. Val-3-APG

Statistically different groups: A. valine p-nitrobenzyl ester, B. Val-3-HPG, C. valacyclovir, Val-OBn; D. Val-3-APG

Statistically different groups: A. Phe-3-HPG, Phe-OBn; B. Phe-OMe, Phe-OEt

Statistically different groups: A. Phe-3-HPG, Phe-OBn, B. Phe-OMe, C. Phe-OEt