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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Sep 17;1808(1):352–359. doi: 10.1016/j.bbamem.2010.09.007

Fig. 7. Effect of curcumin on sLDLr binding of HDL.

Fig. 7

HDL without or with curcumin (5 μg protein) were incubated with 5 μg of sLDLr in 25 mM Tris-HCl, pH 7.4, 140 mM NaCl, 27 mM KCl, 2 mM CaCl2 for 16 h at 4 °C, followed by IP with anti-c-Myc-Agarose. sLDLr-bound apoE was detected by Western blot using HRP-conjugated polyclonal apoE antibody. The lane assignments are as follows: Lane 1, HDL with no curcumin; lane 2, HDL with curcumin; lane 3, no HDL. Lanes 4 and 5 represent Western blot controls containing HDL (1 μg) without and with curcumin, respectively.