Table 1.
Spota | ASQb |
Protein function/namec Source is italicized |
Genec | NCBI Accession # | Theoreticald |
Se | Mf | Cg | Fh | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | B | C | D | Mr | pI | ||||||||
Iron acquisition/Virulence | |||||||||||||
22 | Outer membrane receptor for ferrienterochelin and colicins A. baumannii ACICU |
fepA | gi|184157262 | 82767 | 5.66 | 172 | 26 | 39 | TC | ||||
27 | Ferric aerobactin receptor protein A. baumannii ATCC 19606T |
gi|260555123 | 85609 | 7.55 | 346 | 42 | 74 | TC | |||||
31 | Ferric anguibactin receptor A. baumannii ATCC 19606T |
bauA | gi|260556700 | 82960 | 5.86 | 288 | 28 | 54 | TC | ||||
33 | Ferric aerobactin receptor protein A. baumannii ATCC 19606T |
gi|260555123 | 85609 | 7.55 | 138 | 25 | 38 | OM | |||||
37 | Ferric enterobactin receptor precursor A. baumannii AYE |
fepA | gi|169796822 | 82746 | 5.66 | 146 | 23 | 42 | OM | ||||
39 | TonB-dependent vitamin B12 receptor A. baumannii ATCC 19606T |
btuB | gi|260557148 | 68121 | 5.22 | 277 | 28 | 57 | OM | ||||
45 | FhuE receptor A. baumannii ATCC 19606T |
fhuE | gi|260555105 | 80612 | 5.44 | 98 | 10 | 14 | OM | ||||
46 | FhuE receptor A. baumannii ATCC 19606T |
fhuE | gi|260555105 | 80612 | 5.44 | 226 | 25 | 42 | OM | ||||
47 | Ferric anguibactin receptor A. baumannii ATCC 19606T |
bauA | gi|260556700 | 82960 | 5.86 | 242 | 31 | 48 | OM | ||||
48 | Outer membrane receptor protein, Fe transport A. baumannii ATCC 19606T |
gi|260557370 | 78171 | 5.47 | 339 | 33 | 58 | OM | |||||
49 | Ferric enterobactin receptor precursor A. baumannii AYE |
fepA | gi|169796822 | 82746 | 5.66 | 113 | 19 | 28 | OM | ||||
53 | Ferric aerobactin receptor protein A. baumannii ATCC 19606T |
gi|260555123 | 85609 | 7.55 | 356 | 39 | 68 | OM | |||||
54 | Outer membrane receptor for ferrienterochelin and colicins A. baumannii ACICU |
fepA | gi|184157262 | 82767 | 5.66 | 186 | 28 | 46 | OM | ||||
55 | Ferric aerobactin receptor protein A. baumannii ATCC 19606T |
gi|260555123 | 85609 | 7.55 | 405 | 48 | 73 | OM | |||||
57 | Ferric anguibactin receptor A. baumannii ATCC 19606T |
bauA | gi|260555123 | 85609 | 7.55 | 343 | 33 | 62 | OM | ||||
Porins | |||||||||||||
35 | Carbapenem-associated resistance protein A. baumannii ATCC 19606T |
carO | gi|260556565 | 26855 | 4.66 | 133 | 12 | 76 | OM | ||||
Adaptation/Stress tolerance | |||||||||||||
26 | Fumarase C (fumarate hydratase class II) A. baumannii ATCC 19606T |
fumC | gi|260554730 | 50111 | 5.74 | 128 | 17 | 39 | TC | ||||
Energy/Metabolisms | |||||||||||||
4 | Serine hydroxymethyltransferase A. baumannii AYE |
glyA | gi|169795307 | 44967 | 5.44 | 107 | 11 | 36 | TC | ||||
36 | F0F1 ATP synthase α subunit A. baumannii ATCC 17978 |
atpA | gi|162286757 | 55363 | 5.29 | 207 | 25 | 45 | OM |
Average spot quantity per treatment group (n = 3); columns A, −Fe/−DIP; B, +Fe/−DIP; C, −Fe/+DIP; D, +Fe/+DIP.
Protein function, name and gene were determined by http:/www.ncbi.nlm.nih.gov/BLAST/.
Theoretical molecular mass (Mr) and isoelectric point (pI) were calculated by http:/www.expasy.org/.
Mascot score.
Number of matched peptide masses.
Percent sequence coverage.
Protein fraction, total cell (TC) or Outer membrane-enriched (OM) protein extracts.