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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Sep 29;1808(1):482–489. doi: 10.1016/j.bbamem.2010.09.017

Figure 3.

Figure 3

Refolding of purified Ail. (A) MALDI mass spectrum of purified Ail. The observed major peak at 17,810.55 m/z corresponds to the calculated molecular weight of 15N-labeled Ail. (B) SDS-PAGE showing: Ail folded in 14-O-PC (Lane 1); Ail dissolved in 8 M urea (Lane 2); Ail folded in 100 mM DHPC (Lane 3); Ail folded in 100 mM DHPC/10 mM DMPC (Lane 4). Unfolded (U) and folded (F) protein migrate at apparent molecular weights near 20 kD and 18 kD, respectively. Samples were loaded without boiling. (MW) Molecular weight markers.