Figure 5. PA co-localizes partially with transferrin.
PA (25 μM) was incubated along with fluorescein-labeled transferrin (0.77 mg/ml) for 4 hours in the presence of SJSA-1 cells. Arrows denote vesicles containing both PA and transferrin and arrowheads point to vesicles containing only one of the labeled entities. The images in the lower row confirm the absence of light leakage from different filters (the microscope settings are identical for the two rows).