Effect of p35 knockout on dopaminergic neurotransmission in acute striatal and prefrontal cortical slices. (A) Immunoblot analysis of phospho-Ser845 GluR1 in striatal slices from control and p35−/− mice treated with SKF-81297 (1 μM, 5min). Blots were probed for phospho-Ser845, and total GluR1. Quantitation of the phosphoprotein, total protein, and the phospho/total protein ratio ± standard error normalized to wild-type controls are shown (*p < 0.05, **p < 0.01, Student’s unpaired t test, n = 4-6 per group). (B) Immunoblot analysis of phospho-Thr34 DARPP-32 in striatal slices. Blots were probed for phospho-phospho-Thr34 of DARPP-32, and total DARPP-32 (**p < 0.01, Student’s unpaired t test, n = 4-6 per group). (C) Immunoblot analysis of phospho-Ser845 GluR1 in acute prefrontal cortical slices (*p < 0.05, Student’s unpaired t test, n = 5-9 per group).