Table 1.
CLRa | RSb | 5′Primer 3′Primer |
---|---|---|
human | ||
ASGR2 NM_001181.3 |
E/Nc | 5′-GCGGGGGAATTCGCCACCATGGCCAAGGACTTTCAAGATATCCAGCAGCTG-3′ 5′-CCCCGGGCGGCCGCTCAGGCCACCTCGCCGGTGGCATTCCGCCT-3′ |
BDCA-2/CD303/CLEC4C NM_130441.2 |
E/N | 5′-GCGGGGGAATTCGCCACCATGGTGCCTGAAGAAGAGCCTCAAG-3′ 5′-CCCCGGGCGGCCGCTTATATGTAGATCTTCTTCATCTTGC-3′ |
CD301/CLEC10A NM_182906.2 |
E/N | 5′-GCGGGGGAATTCGCCACCATGACAAGGACGTATGAAAACTTCCAGTACTTGG-3′ 5′-CCCCGGGCGGCCGCTCAGTGACTCTCCTGGCTGGTCTGACCCAGGC-3′ |
CLEC4D/CLECSF8 NM_080387.4 |
E/N | 5′-GCGGGGGAATTCGCCACCATGGGGCTAGAAAAACCTCA-3′ 5′-CCCCGGGCGGCCGCCTATTCAATGTTGTTCCAGGTATTTTAC-3′ |
DCAL-1/CLECL1 NM_172004.2 |
E/N | 5′-GCGGGGGAATTCGCCACCATGGTTAGTAATTTCTTCCATGTCATACAAGTATTC-3′ 5′-CCCCGGGCGGCCGCCTAAATGTTAAATCTCACCATAGCAGTAATGT-3′ |
DCAL-2/MICL/CLEC12A NM_138337.5 |
E/N | 5′-GCGGGGGAATTCGCCACCATGTCTGAAGAAGTTACTTATGCAGATCTTCAAT-3′ 5′-CCCCGGGCGGCCGCTCATGCCTCCCTAAAATATGTAGAACCAAGC-3′ |
DCIR/CLECSF6 NM_016184.3 |
H/N | 5′-GCGGGGAAGCTTGCCACCATGACTTCGGAAATCACTTATGCTGAAG-3′ 5′-CCCCGGGCGGCCGCTCATAAGTGGATCTTCATCATCTCACAAAC-3′ |
DC-SIGN/CD209 NM_021155 |
E/N | 5′-GCGGGGGAATTCGCCACCATGAGTGACTCCAAGGAACCA-3′ 5′-CCCCGGGCGGCCGCCTACGCAGGAGGGGGGTTTGGGGTGG-3′ |
DC-SIGNR/CLEC4M NM_014257.4 |
E/N | 5′-GCGGGGGAATTCGCCACCATGAGTGACTCCAAGGAACCAAG-3′ 5′-CCCCGGGCGGCCGCCTATTCGTCTCTGAAGCAGGCTGC-3′ |
DECTIN-1/CLECSF12/CLEC7A NM_197947.2 |
E/N | 5′-GCGGGGGAATTCGCCACCATGGAATATCATCCTGATTTAGAAAATTTG-3′ 5′-CCCCGGGCGGCCGCTTACATTGAAAACTTCTTCTCACAAATACT-3′ |
DECTIN-2/CLECSF10 NM_001007033.1 |
B/N | 5′-GCGGGGGGATCCGCCACCATGATGCAAGAGCAGCAACC-3′ 5′-CCCCGGGCGGCCGCTCATAGGTAAATCTTATTCATCTCAC-3′ |
DNGR-1/CLEC9A NM_207345.2 |
E/N | 5′-GCGGGGGAATTCGCCACCATGCACGAGGAAGAAATATA-3′ 5′-CCCCGGGCGGCCGCTCAGACAGAGGATCTCAACGCATACT-3′ |
LANGERIN//CLEC4K/CD209 NM_015717.2 |
Nh/N | 5′-GCGGGGGCTAGCGCCACCATGACTGTGGAGAAGGAGGC-3′ 5′-CCCCGGGCGGCCGCTCACGGTTCTGATGGGACTAAGGGT-3′ |
MINCLE/CLEC4É/CLECSF9 NM_014358.2 |
H/N | 5′-GCGGGGAAGCTTGCCACCATGAATTCATCTAAATCATCTG-3′ 5′-CCCCGGGCGGCCGCTTAAAGAGATTTTCCTTTGTTCAAAG-3′ |
NKG2A/CD159A/KLRC1 NM_002259.4 |
E/N | 5′-GCGGGGGAATTCGCCACCATGGATAACCAAGGAGTAATCTACTCAGACCTG-3′ 5′-CCCCGGGCGGCCGCCTAAAGCTTATGCTTACAATGATATATTATTGA-3′ |
NKG2D/CD314/KLRK1 NM_007360.2 |
E/N | 5′-GCGGGGGAATTCGCCACCATGGGGTGGATTCGTGGTCG-3′ 5′-CCCCGGGCGGCCGCTTACACAGTCCTTTGCATGCAGATGT-3′ |
mouse | ||
CD209a/Cire/DC-Sign NM_133238.5 |
E/N | 5′-GCGGGGGAATTCGCCACCATGAGTGATTCTAAGGAAATGGGGAAGA-3′ 5′-CCCCGGGCGGCCGCTCACTTGCTAGGGCAGGAAGTTGAAAG-3′ |
Clec1b/Clec2 NM_019985.2 |
E/N | 5′-GCGGGGGAATTCGCCACCATGCAGGATGAAGATGGGTATATCACTTTAAA-3′ 5′-CCCCGGGCGGCCGCTTAAAGCAGTTGGTCCACTCTTGTCATGCCA-3′ |
Clec4g NM_029465.3 |
E/N | 5′-GCGGGGGAATTCGCCACCATGAACACTGGTGAATACAACAAGC-3′ 5′-CCCCGGGCGGCCGCTTAGTAGCAACTGCTCCTCTTCTCAC-3′ |
Dcar/Clec4b1 NM_027218.2 |
E/N | 5′-GCGGGGGAATTCGCCACCATGGTTCAGGAAAGACAGCTACAAGGGA-3′ 5′-CCCCGGGCGGCCGCTCATAAGTTTATTTTCTTCATCTGACA-3′ |
Dcir1/Clec4a2/ClecSF6 NM_001170333.1 |
E/N | 5′-GCGGGGGAATTCGCCACCATGGCTTCAGAAATCACTTAT-3′ 5′-CCCCGGGCGGCCGCTCATAAGTTTATTTTCTTCATCTGACA-3′ |
Dcir2/Clec4a4 NM_001005860.1 |
E/N | 5′-GCGGGGGAATTCGCCACCATGGCTTCAGAAATCACTTATGCAG-3′ 5′-CCCCGGGCGGCCGCTCATAAGTATATTTTCTTCACCTGAC-3′ |
Dcir3/Clec4a3 NM_153197.3 |
E/N | 5′-GCGGGGGAATTCGCCACCATGTTTTCAGAAAACATTTATGTTAAC-3′ 5′-CCCCGGGCGGCCGCTCATAAGTATATTTTTTTCACATGGC-3′ |
Dcir4/Clec4a1 NM_199311.1 |
E/N | 5′-GCGGGGGAATTCGCCACCATGGCATTACCAAACATTTATACTGACGTG-3′ 5′-CCCCGGGCGGCCGCTCATACATAGAGCTGCCTCATCTCACAAATC-3′ |
Dectin-2/ClecSF10/Clec4n NM_020001.1 |
E/N | 5′-GCGGGGGAATTCGCCACCATGGTGCAGGAAAGACAATCC-3′ 5′-CCCCGGGCGGCCGCTCATAGGTAAATCTTCTTCAT-3′ |
Dngr-1/Clec9a NM_172732.2 |
E/N | 5′-GCGGGGGAATTCGCCACCATGCATGCGGAAGAAATATATACCT-3′ 5′-CCCCGGGCGGCCGCTCAGATGCAGGATCCAAATGCCTTCTT-3′ |
CD161/NKRP1/Klrb1b NM_001159904.1 |
E/N | 5′-GCGGGGGAATTCGCCACCATGGATTCAACAACACTGGTC-3′ 5′-CCCCGGGCGGCCGCTCAGGAGTCATTACACGGGGTTTCA-3′ |
Siglec-E/Siglecl1/CD170 NM_031181 |
E/N | 5′-GCGGGGGAATTCGCCACCATGCTGCTGTTGCTGCTGCTG-3′ 5′-CCCCGGGCGGCCGCTCATGGCCATGCGGTCCTTTG-3′ |
CLR: C-type lectin receptor,
RS: restriction site,
indicates accession ID of corresponding cDNAs,
E/N: EcoRI/NotI,
H/N: HindIII/NotI,
B/N: BamHI/NotI,
Nh/N: NheI/NotI