Table 1. Survival and DNA sequence analysis of plasmids carrying hydrocarbon chain inserts.
| GP21-M3 -(CH2)3- | GP51-M3 -(CH2)3- | GP21-M12 -(CH2)12-insert | GP51-M12 -(CH2)12- | |
|---|---|---|---|---|
| Survival of cells harboring plasmid, % | ||||
| E. coli strain | ||||
| Wild type | 3.8 (±2) | 2.1 (±0.9) | 4.5 (±0.4) | 0.9 |
| ΔumuDC | 0.05 | 0.06 | 0.05 | 0.05 |
| ΔdinB | 2.7 | ND | ND | 0.6 |
| Number of occurrences | ||||
| DNA sequence | ||||
| Base additions | ||||
| AA | - | - | 4 | 1 |
| AT | - | - | - | 1 |
| A | 29 | 20 | 10 | 4 |
| G | 5 | - | - | - |
| C | - | - | - | 1 |
| Deletions | ||||
| M | - | 1 | 1 | 49 |
| M + 1 | - | - | 21 | 1 |
| M+3 to M+7 | - | - | - | 1 |
| Complex deletions | - | - | 4 | 1 |
| Total colonies analyzed | 34 | 21 | 40 | 59 |
The in vivo analysis was performed by introducing the gapped plasmid carrying the hydrocarbon chain [(CH2)3 or (CH2)12] into UV-irradiated E. coli ZTR10 (wild type), E. coli WBY100 (ΔumuDC), or E. coli AR30 (ΔdinB) cells. Survival was calculated by dividing the number of transformants by that obtained with gapped plasmid without the lesion. The DNA sequence opposite the lesion obtained for individual E. coli ZTR10 clones is shown, transformed with the indicated gap-lesion plasmid. M, hydrocarbon insert, ND, not determined.