Table 2. DNA sequence analysis of products synthesized during in vitro replication across hydrocarbon chains by IV and D pol V holoenzyme.
| GP21-M3 -(CH2)3-
|
GP21-M12 -(CH2)12-
|
GP51-M12 -(CH2)12-
|
||||
|---|---|---|---|---|---|---|
| Pol IV | Pol V | Pol IV | Pol V | Pol IV | Pol V | |
| Number of occurrences | ||||||
| DNA sequence | ||||||
| Base additions | ||||||
| AA | - | - | - | - | - | 1 |
| GA | - | - | - | 1 | - | - |
| AT | - | - | - | - | - | - |
| A | - | 16 | 3 | - | 1 | 8 |
| G | - | 10 | - | - | - | - |
| T | - | - | - | - | - | 1 |
| C | - | - | - | - | - | - |
| Deletions | ||||||
| M | - | 1 | - | 2 | - | 22 |
| M+1 | 19 | - | 3 | 11 | 13 | 2 |
| M+2 | - | - | - | 1 | 2 | - |
| M+3 to M+7 | - | - | 12 | 1 | 4 | 2 |
| Complex deletions | - | - | 15 | 6 | 30 | 20 |
| Others | - | - | - | 1 | - | - |
| Total colonies analyzed | 19 | 27 | 33 | 23 | 50 | 56 |
DNA sequence analysis of products synthesized in vitro by pol IV and pol V was conducted by transforming an E. coli JM109 recA strain with the DNA synthesis reaction products. The DNA sequence opposite the lesion obtained for individual clones is shown. Complex deletions refer to deletions accompanied by at least one additional mutation.