FIGURE 1.

Detection of systemic anti-col(V) or anti-HEL Abs. Serum was collected from rats there were immunized with col(V) or HEL and incubated with Sepharose beads coated with col(V) or HEL. Flow cytometry was used to detect IgG Abs bound to col(V) or HEL-coated beads. Data shown are derived from serum collected from an individual rat immunized with col(V) or HEL and are representative of n = 5–6 rats immunized with each Ag.