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Confocal microscopy expression of col(V) on apical but not basal surface of airway epithelial cells. Primary rat airway epithelial cells were stained for col(V) (Rhodamine red), type IV collagen (col(IV) FITC, green), and nuclei identified by DAPI (blue) staining. Confocal imaging was used to scan sections from apical (left) to basal (right) surface as shown. Data are representative of three separate experiments with similar results.
