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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Cell Signal. 2010 Sep 17;23(1):213–221. doi: 10.1016/j.cellsig.2010.09.005

Figure 5. Constitutively GDP-bound and mutationally activated Gα12 bind PC1 in vitro with similar affinity.

Figure 5

For panels A-C, cellular expression of proteins and in vitro interaction assays were performed as described in 2.4. (A) Comparison of G228Aα12 (GAα12) with QLα12 in pulldowns using GST fusions of the PC1 C-terminus and the Aα subunit of PP2A. (B) Binding of G228Aα12 and QLα12 to GST-LARG. (C) Quantification of relative binding of G228Aα12 to each partner, compared with QLα12. (D) Apoptosis of HEK293 Cells +/− PC1 overexpression, transiently transfected with G228Aα12, wildtype Gα12 or QLα12. Cells were stimulated +/− thrombin (2 U/ml) for 24 h prior to determining apoptosis as described in Material and Methods.