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. 2010 Oct 4;285(50):39425–39436. doi: 10.1074/jbc.M110.149500

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — MALDI-TOF analysis of Ste2p-Bio-DOPA¹ cross-linked CNBr fragment. A, immunoblot analysis of Ste2p-Bio-DOPA¹ cross-linked sample after CNBr treatment. The samples were resolved on 4–20% gradient SDS-PAGE and probed with anti-FLAG to detect Ste2p and with Na-HRP to detect the biotin tag on Bio-DOPA¹-α-factor. A band at about 7 kDa is observed in the Bio-DOPA¹-treated lane. B, MALDI-TOF spectrum of fragmented Ste2p- Bio-DOPA¹ cross-linked to peptide eluted from avidin resin. A 6571.883-Da peak corresponding to Ste2p fragment Ser²⁵¹–Met²⁹⁴ (4572.455 Da) cross-linked to Bio-DOPA¹ α-factor (1998.221 Da) was observed. C, spectrum of sample eluted from avidin mixed with CNBr-treated Ste2p (without cross-linking).