FIGURE 1.

ROS regulate migration of human lung epithelial H460 cells. A, confluent monolayers of H460 cells were wounded, and the cells were allowed to migrate for 24 h in the presence or absence of various ROS modulators, including MnTBAP (50 μm), DMNQ (5 μm), CAT (7,500 units/ml), H₂O₂ (100 μm), NaFM (5 mm), and FeSO₄ (50 μm). Wound space was visualized under a phase contrast microscope and analyzed by comparing the relative change in wound space of the treated over nontreated cell monolayers. Representative micrographs from four independent experiments are shown. B, effect of ROS modulators on cell viability. Cell monolayers were similarly treated with the indicated concentrations of ROS modulators and analyzed for cell viability after 24 h by MTT assay. Data are the mean ± S.D. (n = 4). *, p < 0.05 versus nontreated control.